Roma Tauler Federico Marini

Ion-Mobility Mass Spectrometry and Applications

Program Highlights:

  • Structural Mass Spectrometry Analysis
  • Basics of Ion Mobility-Mass Spectrometry
  • Ion-Mobility Mass Spectrometry for Small Molecule Analysis
  • Structural Characterization of Supramolecular Complexes and Assemblies using Ion-Mobility Mass Spectrometry
  • Ion Mobility-Mass Spectrometry Analysis for Native Biomolecule Characterization
  • Monitoring Noncovalent Complexes and Interactions using Ion-Mobility Mass Spectrometry
  • Collision Cross Section Calculation
  • Data Evaluation and Reporting Strategies for Ion-Mobility Mass Spectrometry Measurements

High and ultrahigh resolution Mass Spectrometry

Program Highlights:

  • The challenge of complex mixtures analysis
  • The concept of hyperfine isotopic structure
  • Isotopic analysis without degrading the molecule to simple gas
  • Quantitative analysis in bottom-up proteomic by neutron encoding
  • Kendric and Van Krevelen diagram
  • Petroleomic
  • Metabolomic analysis by direct infusion MS: acquisition and data treatment
  • Comparison between LC/MS approach and direct infusion MS: pros and cons
  • Two dimensional (2D) MS
  • Evolution of the isotopic distribution with the mass of the parent structure
  • Top-down proteomic characterization of posttranslational modifications
  • How to get access to an ultrahigh resolution MS: the EU_FT-ICR_MS network (an EU Horizon 2020 funded Research Infrastructure, grant agreement 731077)

Target Audience:

In the ion mobility-mass spectrometry part of the course, the fundamentals of ion mobility-mass spectrometry and its applications will be given in detail. The structural analysis of small molecules such as drugs or metabolites, as well as the applications of analyzing the conformational features of macromolecules such as proteins, lipids, polymers and synthetic supramolecular complex structures using ion mobility-mass spectrometry will be discussed. The usefulness of ion mobility-mass spectrometry technique will be explained by what kind of structural data and information can be obtained about the nature of interactions between different species in the gas phase. Recommendations for the evaluation and presentation of data obtained from ion mobility-mass spectrometry analyzes will be discussed.

The high and ultrahigh resolution mass resolution will focused on the new opportunities afforded to the analytical scientist by these resolutions, which are now routinely accessible on Fourier Transform Mass Spectrometers (Orbitrap®, FT-ICR or MRMS®). The course will start by introducing the concept of hyperfine isotopic structure and the tools for exploiting the mass spectra containing thousands of peaks the Kendrick and Van Krevelen. These concepts will be illustrated by a journey through applications in different fields (isotopic analysis, petroleomic, metabolomic, top and bottom-up proteomic). Finally a way to get access to ultra high resolution through the EU_FT-ICR_MS network (an EU Horizon 2020 funded Research Infrastructure, grant agreement 731077) will be presented.

This course is intended for researchers from different disciplines who are still working or having future study plans on the structural characterization of molecules and their interactions.