Sample preparation is an often overlooked but extremely important aspect of any analytical method. Solid-phase microextraction (SPME) is a simple, fast, sensitive, and environmentally-friendly equilibrium-based sample preparation technique that allows the integration of sampling and sample preparation steps. Due to these unique features, SPME is considered one of the six “Great Ideas of a Decade” by ACS Analytical Chemistry journal. This course will cover both basic and advanced SPME topics with the focus on the main principles of SPME including thermodynamic and kinetic theory, calibration methods and coupling strategies of SPME to GC-MS and LC-MS. A complete method development strategy will be described and illustrated in detail by real-life examples. A variety of SPME applications in the fields such as forensic, environmental, food and beverage, industrial hygiene and clinical analysis will be presented. The discussion will include the comparison of the performance of SPME to various established methods relevant for particular application. Recent advances in SPME will be highlighted including (1) on-site sampling with thin film SPME (2) the use of SPME for ligand-receptor binding and plasma protein binding studies (3) the use of SPME to perform in vivo sampling of freely moving animals (4) automation of SPME in 96-well plate format for applications requiring high-throughput (5) the use of SPME for direct tissue sampling and medical applications and (5) direct coupling to mass spectrometry.
The course is targeted at both new and current SPME users. The primary goal of the course is to provide the users of the technique with deeper insight into the main principles of this technique and thus increase their productivity and the quality of analytical results. This course will be of interest to analytical chemists, laboratory supervisors, scientists and industry regulators in the environmental, food and beverage, pharmaceutical, clinical, cosmetic, industrial hygiene and many other fields. The unique features of in vivo SPME sampling will be of particular interest to researchers in life sciences.
- Introduction to SPME
- Theoretical principles of SPME
- SPME interfaces to analytical instrumentation including MS
- Needle trap and thin film microextraction and on-site sampling
- Calibration of SPME
- SPME method development
- Automation of SPME
- In vivo sampling using SPME
- Pharmaceutical, clinical and medical applications of SPME
- Food, flavor and fragrance applications of SPME