Monday, September 2nd, 2019

 

 


Emeric Gueneau
Biacore Application Specialist, GE Healthcare

“To Affinity and Beyond : Biomolecular interaction analysis using Surface Plasmon Resonance”

Abstract:
Understanding the nature of interactions between molecules is fundamentally important for increased understanding of biological processes. BiacoreTM surface plasmon resonance (SPR)-based sensors are widely used to study molecular interactions in real-time and are applied as powerful tools in research and drug discovery throughout to quality control and drug-release testing. The SPR technology, which measure real-time analysis of biospecific interactions without the use of labeled molecules, have since the introduction constantly been refined and today there are practically no limitations on which molecular interactions can be studied, ranging from low molecular weight compounds to large particles, even against challenging targets.

In particular for the drug discovery process of both traditional drugs and protein biotherapeutics, BiacoreTM  systems are now standard tools for the screening to characterization process by providing detailed and relevant high content data information about the drug candidate binding characteristics. Also, in later drug development stages SPR is becoming an essential tool for e.g. safety and quality control testing.

The lecture will describe the use of and the information obtained with automated, high-throughput BiacoreTM systems & present an overview of target molecules that have been studied using BiacoreTM technology. Further, emerging application areas for BiacoreTM within the drug discovery and process development field will be discussed in the context of the unique benefits and potential challenges for the SPR technology in the future.

 




Wesam AbuSaif
CAS Specialist

“Chemical Abstracts Service collective data to find the best analytical methods”

MethodsNow is one of the latest Chemical Abstracts Service product to solve an important problem that many analytical chemists are facing which is developing the best analytical method for their analyte. Chemical Abstracts Service scientists went back to all the analytical methods published after year 2000 and rewrite the analytical methods in a protocol form. I Will give some historical background about Chemical Abstracts Service and I will show some examples of how to find very good methods in few minutes.

Tuesday, September 3rd, 2019

Vladimir Ioffe, Ph.D.
Associate Director, Scientific Expert, Analytical Development, Global R&D

In this seminar, specific problems related to gradient RP HPLC methods will be discussed, as well as the ways of solving them.
The first problem is the most common: the influence of dwell volume mismatch between different instruments, which results in difficulties in transfer of gradient methods. This problem is illustrated with typical examples of why some brilliant methods are hard to transfer and how to solve such issues in intra- and interlaboratory studies.
Various types of gradient equipment are presented and the simple method of dwell volume determination is explained.
The second problem is the gradient shape: how accurate is the mixer and what is the reason that in the gradient methods retention time drift may be different in the different segments of the chromatogram. Simple but efficient methods of testing of functioning of the mixers in the gradient chromatographic systems to ensure the proper gradient shape are explained.
The third problem is the gradient artifact peaks. This is the most complicated part of the problems associated with baseline issues.
The “components” of gradient baseline shape are analyzed, including their influence on analytical result. The main aim of this part is to learn how to diagnose gradient artifact peaks and how to get rid of them. The questions of quality of solvents and reagents, as well as the ways of HPLC systems cleanup are in the focus of this topic.

Dr. Laura Fernández Llano,
Product Manager

Combining Spectroscopy & Electrochemistry produces powerful results

Spectroelectrochemistry (SEC) is a powerful electrochemical research tool that allows you to simultaneously change electrochemical reaction conditions and monitor these changes with spectroscopy. This hyphenated technique is especially useful to those in the disciplines of materials science, biotechnology, pharma, corrosion, energy and fundamental research exploring interfacial reactions and redox processes.

Recent developments in instrumentation have made SEC more accessible. Integrated systems combine the best features of electrochemistry with UV-VIS, Near IR or Raman spectroscopy while reducing the need for comprehensive expertise of each technique.

During seminar, Dr. Laura Fernández, Product Manager at Metrohm DropSens will introduce the electrochemical and spectroscopic measurements possible with SEC, including Raman SEC and NIR SEC experimental results. MSc Yeliz Yavuz Çevik, Electrochemistry Product Manager at Metrohm Turkey, will run an experiment where UV-VIS SEC is shown.  A question and answer session will help you personalize this information to improve your lab’s capability. 

Dr. Murat Emrah Maviş
Sem Laboratuar Cihazları Pazarlama San. ve Tic. Inc   
R & D Center, Istanbul, TURKEY

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) in The Analysis of Biomarkers-Quantitation of Phosphatidylethanol (PEth 16:0/18:1) in DBS Samples

This lecture will provide insights into LC-MS/MS analysis of clinically relevant biomarkers. Many clinical strategies are based on treatment decision which are driven by quantitative results obtained from the analysis of biological materials. Given the potential of such molecules, it is important to identify and quantify targeted biomarkers by implementing reliable analytical methods. Thanks to ability of rendering robust, reliable, and economical methodology in clinical quantitative targeted analysis, LC-MS/MS smooths the way of diagnosis of inherited metabolic disorder and evaluation the patient’s compliance with drug therapy and misuse or abuse of prescribed/non-prescribed medications into clinical routine applications. Besides, Phosphatidylethanol (PEth), blood-based direct alcohol biomarker, represents a group of unnatural phospholipids formed on leukocytes and predominantly erythrocyte membranes. The presence of Peth 16:0 18:1 in blood samples is an indisputable indicator of ethanol intake. DBS has been gaining interest in plenty of research areas owing to ease of specimen collection and storage. To overcome challenges coming from blood viscosity, volumetric DBS devices have begun powerful alternative for specific volume of blood collection.

 

Wednesday, September 4th, 2019

 


Ing. Rainer Rozenich
Separations – Business Development Manager
Eastern European Region

“Analytical Method Life Cycle Management“
 
During this presentation you will learn about the concept of analytical method life cycle management based on a stimuli article for the U.S. Pharmacopeia.
The proposed new chapter “The Analytical Procedure Lifecycle ⟨1220⟩” explains how the implementation of a Quality by Design approach in method development ultimately leads to a better understanding of your test methods. You will know how to define the analytical target profile –the starting point in method development, and how the new concept will add flexibility to the method for future upcoming changes. Such changes could be of a major or minor classification. The supposed chapter 1220 also addresses how to cope with them in a regulated environment.

https://www.waters.com/waters/eventInstance.htm?eiid=135026614

phenomenex ile ilgili görsel sonucu


Frédéric Thiebaut

"Useful Approaches to LC and GC Method Development"

Abstract: 

The presentation is split in two parts. The fist part is focusing on LC and the second part is focusing on GC.

You will learn the influence of critical parameters in LC and GC Method development. You will learn how these parameters are linked to specific properties of the column and stationary phase. Finally, you will learn how to chose the correct column and stationary phase for your analytes based on the properties of your analytes and their sample matrix.